The talk show host regained the title this year, earning $315 million over the past 12 months. Winfrey is set to launch her own television channel next year.
She bumped Angelina Jolie from the top spot. The actress slipped to number 18. Singer Beyonce Knowles came in second. James Cameron, the director of the record-breaking movie Avatar came in at number three.
Lady Gaga and Tiger Woods rounded out the top five.