San Francisco Mayor Ed Lee and New Orleans Mayor Mitchell Landrieu each put their city's signature food and drinks on the line for the game, which is being played at 1:35 p.m. Saturday at Candlestick Park.
If the 49ers win, Landrieu will send fresh Louisiana seafood, a Sucre king cake and Abita beer to Lee.
But if the Saints win, Lee will have to send over some San Francisco seafood, including Dungeness crab from Fisherman's Wharf, as well as Boudin sourdough bread and Anchor Steam beer.
"Our 49ers are a source of pride for San Francisco and the entire Bay Area," Lee said in a statement. "While I'm excited to make this bet with Mayor Landrieu, we have no intention of sending anything to New Orleans. Go Niners!"
Landrieu countered, "We don't expect to be sending any (food) to the Bay City anytime soon. We look forward to sampling Dungeness crab and other San Francisco treats next week."
Lee and Landrieu will be among city heads who will visit Washington, D.C., next week for the annual U.S. Conference of Mayors.
The last time a San Francisco pro sports team made a playoff run, the city's mayor made a killing in his official wagers.
As the San Francisco Giants made their way to a World Series title in 2010, then-Mayor Gavin Newsom and his staff sampled some of the finest foods from Atlanta, Philadelphia, and Arlington, Texas, after Newsom won bets against the mayors of those cities.