The vaccine was given to the zoo's highest risk animals on Thursday, including tigers, black bears, grizzly bears, mountain lions and ferrets.
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Chimpanzees, fruit bats and pigs are next to receive the vaccine.
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A total of 11,000 doses of the vaccine were donated to zoos across the country by Zoetis, a company that manufactures drugs for animals and livestock.
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The COVID-19 vaccine for animals has been authorized for experimental use by the USDA.