Novato police say Around 100 people participated in the rally. The demonstration ended just before 7p.m.
People were holding American flags and President Trump signs.
The California Highway Patrol closed the off ramp at the Delong Avenue exit in the Northbound direction.
Traffic moved slowly on Highway 101 through the area.
A similar show of support appeared in the same spot last month.
There were no arrests or citations.
WATCH: Supporters of President Trump are seen waving flags on an overpass in Novato
Crowd shuts down overpass in Novato, shows support for President Trump