The crash occurred at Melita Road shortly before a Sig-alert was issued at 6:19 p.m.
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Sheriff's officials said the deputies were responding to a domestic violence call.
One deputy suffered moderate injuries and was taken to a hospital.
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State Highway 12 reopened in both directions on Wednesday evening around 8:05 p.m.
CHP officials said 30 feet of metal guardrail was damaged as well as posts and a road sign.
ABC 7 News contributed to this post