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Kori Ali Muhammad, 39, shouted twice during a minute-long hearing: "Let black people go in reparations."
Muhammad was charged Thursday with killing Carl Williams, an unarmed security guard, at a Motel 6 a week earlier.
Police said that murder was the first of four killings by Muhammad.
On Tuesday, he allegedly went on a shooting spree, killing three other white men.
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At Friday's hearing, Fresno County Superior Court Judge W. Kent Hamlin assigned a doctor to examine Muhammad. Bail was set at $2.6 million.
He's due to return for arraignment on May 12.
Muhammad said Williams had shown him disrespect while Muhammad was visiting a woman at the motel, according to police.
Prosecutors say Muhammad will face murder charges for all four men, but they're waiting for investigators to assemble their case before filing the other three.
Click here for full coverage on the Fresno shooting spree.
ABC 30 contributed to this report.