Ruiz says she believes the manager discriminated against them because they're Latino.
"If I would have been white, I think I would have experienced more privilege," she said. They would have accommodated us in a different car train. And definitely from the other guests, more tolerance."
VIDEO: Apology issued after book club booted off Napa Wine Train
This latest accusation surfaced after a black women's book club was kicked off the train Saturday after passengers complained they were being loud.
But then the group was offended to see a Facebook post from the Wine Train, which has since been deleted. It read in part: "Following verbal and physical abuse towards other guests and staff, it was necessary to get our police involved."
"That is absolutely untrue," Johnson said. "We have never, we never touched anybody."
PR consultant Sam Singer, has been hired as a spokesperson for the Napa Valley Wine Train, he says that post was a mistake.
The group refused an apology from the train company's CEO.
The Napa Valley Wine Train CEO says "We were 100% in the wrong" for the way a Black Woman's book club was treated. pic.twitter.com/lM1Ma1RFWB— Sergio Quintana (@svqjournalist) August 25, 2015
A spokesperson has denied the latest allegation of racial bias.
ABC7 News reporter Sergio Quintana contributed to this story.