The San Francisco native and Lowell High School graduate took part in a comedy bit with Tina Fey and Amy Poehler.
The trio took aim at North Korea's reaction to the movie "The Interview."
Cho wore face paint and spoke with a thick accent, voicing a collection of complaints about Hollywood as well as the Globes: "I think 'Orange is the New Black' should be in Drama category," rather than Comedy, she barked in broken English. "It funny, but not ha-ha funny."
Some people online are calling the skit racist.
Cho responded to the controversy on Twitter, writing: "I'm of mixed North/South Korean descent - you imprison, starve and brainwash my people you get made fun of by me #hatersgonhate #FreeSpeech."
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The Associated Press contributed to this report.