The 26-minute video was posted on militant websites used by al-Qaeda members and its affiliates.
Ayman al-Zawahiri claimed bin Laden would continue to terrify America in death. The al-Qaeda second-in-command blasted the United States for burying bin Laden at sea.
al-Zawahiri urged the people of Pakistan to rise up against the country's military rulers and politicians.
Officials believe al-Zawahiri is hiding somewhere on the frontier border between Pakistan and Afghanistna.