The robbery occurred at about 7:45 p.m. at the business, located at 3487 El Camino Real.
The male suspects, who have not yet been identified, walked into the store wearing masks, flashed a gun and demanded money from the register, according to police.
The store clerk complied and handed them an unspecified amount of cash and the suspects ran away.
Officers responded but were not able to find them.
No one was harmed during the course of the robbery.
The suspects were described as being around 6 feet tall with thin builds. They were wearing dark pants, masks, and black hooded sweatshirts.
Anyone with information about the case is asked to call Palo Alto police at (650) 329-2413.
