The stretch of freeway will be closed between 2 a.m. and 6 a.m. between Airway Boulevard and North Livermore Avenue.
Detour signs will be posted.
Caltrans says there will also be various lane closures on Sunday beginning at midnight and continuing until 9 a.m.
Motorists are advised to expect delays and allow extra time for their commute.
During the closure, crews will be removing a temporary structure that helps support the bridge during construction.